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. Author manuscript; available in PMC: 2015 Jun 5.
Published in final edited form as: J Nat Sci. 2015 Jun;1(6):e111.

Figure 2.

Figure 2

Triton X-100-induced autolysis of the conditional walR and walK mutants. The Pspac-regulated walR mutant, RNPspac-WalR (A) and the Pspac-regulated walK mutant, RNPspac-WalK (B) were grown in TSB in the presence of different concentrations of IPTG (solid square:1 mM; open circle: 10 μM) or in the absence of IPTG to an OD580 of 0.6-0.8. The bacterial cells were harvested by centrifugation and resuspended in the same volume buffer containing 50 mM Tris-HCl (pH 7.5) and 0.1% Triton X-100. The cells were incubated at 30°C with shaking, and the changes of optical densities (OD580) were measured. Results were normalized to OD580 at time zero (OD0). The percent lysis at time t = [(OD0-ODt)/OD0] × 100. The experiments were repeated at least three times. Each figure represents the results of one experiment.