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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Cell Microbiol. 2014 Oct 31;17(2):147–163. doi: 10.1111/cmi.12362

Figure 2. Lm lacking p60 and NamA autolysins exhibit a filamentous phenotype promoting extracellular growth within infected spleens.

Figure 2

(A) Morphology of Lm colonies grown OVN onto BHI Petri dishes, and corresponding gram staining of bacteria. (B–D) WT C57BL/6 (B6) mice were infected with 0.1×LD50 ΔActA (106), ΔSecA2 (0.6×106) and Δp60ΔNamA (0.6×107) Lm; spleen harvested at the indicated times, lysed and plated. In (C), mice received gentamycin i.p. (2 mg) 1 hr after Lm infection. In (D) immunized mice were bled (200µl/mouse), spleens harvested at indicated times, and Lm CFU determined by plating both cell suspensions after lysis. Data plots and histogram bars average mean values+/−SEM (B-D).