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. 2014 Oct 7;1(2-3):126–132. doi: 10.1016/j.ebiom.2014.10.002

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© 2014 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).

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Fig. 5 — MRJ-L but not MRJ-S mediates nuclear import of Vpr. (a) Localization of Vpr (tagged with GFP) co-expressed with HA-tagged MRJ-L or MRJ-S. Cells were fixed and stained with an anti-HA antibody. Bars: 10 μm. (b) Depletion of MRJ-L in HeLa cells by shRNAs shJ10 or shJ8 was verified by western blotting. > 90% knockdown efficiency was achieved by shJ8. (c) Subcellular localization of Vpr (tagged with GFP) in HeLa cells treated with shRNA (Scramble) or shRNA (MRJ-L, shJ8). Vpr was not detected in the nucleus when MRJ-L was depleted. DAPI staining shows the nuclei. Bars: 10 μm.