Skip to main content
. 2015 Jun 5;10(6):e0129302. doi: 10.1371/journal.pone.0129302

Fig 1. Detection of Introner-Like Elements (ILEs) in closely related dothideomycetous fungal species.

Fig 1

(A) Maximum-likelihood phylogenetic tree using ITS and LSU sequences. Fungal species in which ILEs have been previously identified are highlighted in bold. Aspergillus niger belongs to the Eurotiomycetes and serves to root the tree. Accession numbers of fungal species from the CBS-KNAW collection are indicated in between brackets. The scale bar indicates the number of substitutions per site. (B) PCR with primers specific to single (cf01 and cf02) or shared (cf02cf03ds01ds05, cf04ds03 and cf08ds04) ILE families between F. fulva and Dothistroma septosporum was performed using genomic DNA. PCR products were run on 20% acrylamide gels. The first row shows a 50 bp-step DNA ladder and the last row shows the water control. Asterisks indicate fragments that correspond to two different ILEs as revealed by sequencing.