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. 2015 Jun 5;10(6):e0128390. doi: 10.1371/journal.pone.0128390

Fig 3. SDS-PAGE analysis of chimeric proteins, point mutants purified by metal affinity chromatography under denaturing conditions.

Fig 3

A. coomassie-stained gels. B. corresponding anti-His-tag antibody stained imunoblots. The amount of the protein loaded was normalized to the total amount of cell culture. C. The yields of the chimeric proteins and point mutants as quantified by the BCA protein assay, band density on SDS-PAGE and Western blotting. Chimeric proteins containing extracellular N-terminus of SRII have shown a much better yield.