FIGURE 7.

SFK play a critical role in PMN arrest, polarization, and transmigration across inflamed endothelium. a, PMN in the presence of 1 mM Src kinase inhibitor II or 0.3% DMSO control were perfused through a microfluidic flow chamber at an average wall shear stress of 2 dyne/cm² and observed interacting with inflamed HUVEC monolayers. Neutrophils were imaged over the course of 6 min and assayed for firm arrest, polarization, and TEM. +, p < 0.05 and **, p < 0.001. b, Wild-type (WT) or lyn^−/−hck^−/− fgr^−/− (SFK KO) murine bone PMN were perfused over inflamed bEND.3 endothelial cells at an average wall shear stress of 1 dyne/cm². As in a, stable arrest, polarization, and TEM were identified optically. Data are means ± SEM from three independent experiments on murine replicates. *, p < 0.01.