Table 3.

Free-energy decomposition of epitope residues in the four systems

Residue ID	18HA		06HA		09HA		09HA_mut
	Residue	ΔG ± SE	Residue	ΔG ± SE	Residue	ΔG ± SE	Residue	ΔG ± SE
158	K	−4.4 ± 0.2	–	−2.2 ± 0.2	–	−2.3 ± 0.2	–	−1.9 ± 0.2
159	G	−3.1 ± 0.1	N	−2.9 ± 0.1	–	−2.2 ± 0.1	–	−1.8 ± 0.1
160	S	−4.9 ± 0.2	G	−0.0 ± 0.0	N	−0.5 ± 0.1	N	−0.9 ± 0.1
161	S	−1.8 ± 0.1	L	−4.0 ± 0.1	–	−1.8 ± 0.1	–	−2.0 ± 0.1
167	K	−6.6 ± 0.2	–	−6.6 ± 0.2	–	−6.3 ± 0.2	E	2.9 ± 0.1

Only selected key residues—158, 159, 160, 161, and 167—are shown. All epitope-residue-based decomposition results appear in the Supporting Material (Table S8). All units are in kcal/mol.