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. 2015 Jun 2;108(11):2721–2731. doi: 10.1016/j.bpj.2015.04.038

Figure 1.

Figure 1

Molecules used for dye parameterization and testing: (A) AlexaFluor 488 free acid, (B) AlexaFluor 594 free acid, (C) poly-L-proline (shown is polyproline-11 labeled via an N-terminal Gly with Alexa594 and via a C-terminal Cys with Alexa488), (D) N-terminally labeled CspTm (via Cys at position 2, Alexa 488 shown), and (E) C-terminally labeled CspTm (via Cys at position 68, Alexa 488 shown). In (C), the vectors used for transition dipole moments are indicated on the donor and acceptor (rD and rA, respectively), together with the vectors parallel and perpendicular to the polyproline axis (r|| and r⊥, respectively). To see this figure in color, go online.