Figure 3.

Fluorescence membrane binding curve of Myo1C-EGFP in U2OS cells. (A) Fluorescence intensity at the bottom (open triangles) and top (solid triangles) membrane versus fluorescence intensity in the cytoplasm. The fit (dotted line) to Eq. 6 recovers F₀ = 83 kcps and F_K = 24 kcps. (Top and right axes) Cytoplasmic and membrane-bound protein concentrations. (B) Membrane intensity fraction versus fluorescence intensity in the cytoplasm (open triangles) is well approximated by Eq. 8 (dotted line) with the same F₀ and F_K as determined in the top panel. To see this figure in color, go online.