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Fluorescence membrane binding curve (open triangles) for LactC2-EGFP in U2OS cells with fit (solid line) to Eq. 6 (F0 = 530 kcps, FK = 68 kcps). Competitive binding curve for LactC2-EGFP coexpressed with LactC2-mCh at a ratio of 1:3 (open squares) agrees with predicted binding curve (dashed line). To see this figure in color, go online.
