Figure 7.

Slowed activation speed of the probe can also widen the I-X curve (Experiment). (A) In a single cell using a normal probe, the filtering of the stimulating step response was changed to 15, 1, and 200 Hz. Families of step responses were done at each filter setting keeping the position of the probe the same. (B) Corresponding I-X curves for the traces in (A) for 15 (black), 1 (red), and 200 Hz (blue) showing the broadening of the I-X curve at the slowest stimulating speed. (C) Summary of the OR of the I-X curve calculated from the double Boltzmann fit for the different stimulating speeds for four different cells represented with different symbols. The probe rise time was measured for the stimulator. To see this figure in color, go online.