Skip to main content
. Author manuscript; available in PMC: 2015 Jun 5.
Published in final edited form as: Sci Signal. 2015 Feb 10;8(363):ra14. doi: 10.1126/scisignal.2005735

Fig. 5. Ski also destabilizes TAZ through a Lats2-independent mechanism.

Fig. 5

(A) Ski decreased the stability of TAZS89A. Cells transfected with Flag-TAZS89A together with or without Flag-Ski were subjected to a pulse-chase assay. Data in the graph were derived from at least three independent experiments and are presented as means ± SEM (Student’s t test, *P < 0.05). (B and C) Western blotting analysis shows that overexpression of Ski decreased the abundance of TAZS89A (B) and TAZS311A (C). Western blots are representative of two independent experiments. (D) Suppression of TAZ and TAZS89A by Ski required the proteasome. The abundance of endogenous TAZ (left) or TAZS89A (right) was measured in MG132-treated cells expressing Flag-Ski alone (left) or Flag-Ski plus TAZS89A (right) by Western blotting. Western blots are representative of three independent experiments.