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Mass spectrometry of Ni(II) binding to HypA. (Top) HypA was copurified with one equivalent of zinc bound to the protein (Calculated mass: 14273 Da). (Bottom) Upon addition of one equivalent of nickel, the mass of the protein shifts by 59 Da, corresponding to nickel binding (Calculated mass: 14332 Da). In addition to the expected protein peaks, small peaks at approximately +23 Da and +39 Da were observed, corresponding to sodium and potassium adducts.
