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. 2015 Jun 5;10(6):e0128359. doi: 10.1371/journal.pone.0128359

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© 2015 Monaghan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — A. RT-PCR analysis of TRPV4 mRNA expression in cultured bovine retinal microvascular endothelial cells (RMECs). No product was seen when the RT enzyme was omitted (No RT). B(i,ii) Confocal images of RMECs immunostained for TRPV4 (green; ACC-034) and TO-PRO nuclear marker (pseudo-coloured blue) in the absence (i) and presence (ii) of anti-TRPV4 blocking peptide (BP). B (iii) Immunolabelling with a second anti-TRPV4 antibody (SC-98592) revealed a similar pattern of staining in RMECs. Scale bars = 10μm.