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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Biomaterials. 2015 Jan 22;47:1–12. doi: 10.1016/j.biomaterials.2014.12.043

Figure 2. CPC Viability, proliferation, and adhesion in HyA hydrogels.

Figure 2

(a, upper panels) The viability of CPCs encapsulated by the HyA-, HyA-P, HyA-PH and HyA-PHT hydrogels was high after one day of culture, as assessed by double staining with calcein (green, live cells) and propidium iodide (red, dead cells). (a, lower panels) CPCs were capable of adhering and spreading within the hydrogel networks containing the adhesive ligand bsp-RGD(15), as assessed by imaging for f-actin stress fibers (TRITC-phalloidin; red) and nuclei (DAPI; blue). b The area of spread cells was enhanced significantly by the addition of adhesion peptide and TGFβ1. c Cell proliferation within the HyA hydrogels was affected by the presence of heparin and TGFβ1, and at day 21, proliferation in both HyA and HyA-P were significantly less than HyA-PH and HyA-PHT hydrogels (ANOVA with Tukey, p<0.05).