Figure 3. Dependency of cell proliferation on hydrogel stiffness and adhesion peptide density.
a, RSM plots of CPC proliferation in HyA-PHT hydrogels with various moduli and concentrations (120–380 μM) of the cell adhesive ligand bsp-RGD (15). CPC proliferation was dose-dependent on the RGD density, and maximum at ~ 800 Pa. All data was normalized to the cell number (3.48×105) in hydrogels at day 3 data. b, Kinetic observations of CPC proliferation as a function of hydrogel stiffness in HyA-PHT hydrogels shown at three different bsp-RGD(15) densities.