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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Biomaterials. 2015 Jan 22;47:1–12. doi: 10.1016/j.biomaterials.2014.12.043

Figure 5. HyA-PHT hydrogels encouraged angiogenic cytokine expression by CPCs.

Figure 5

a, The concentration of secreted angiogenic factors produced by CPCs and sequestered within HyA-PHT hydrogel after 12 days. To determine the secreted angiogenic factors, hydrogels were degraded by adding the 100 unit/mL of hyaluronidase at 37°C for 6 hr. Subsequently, degraded hydrogels were incubated with heparinase I, II & III at the 2.5 unit/mL concentration of each at 37°C for another 6hr to remove the adhered heparin with the sequestered proteins. These include factors that promote EC proliferation and b, those associated with vascular stability. c & d, The presentation of TGFβ1 affected the production of angiogenic proteins detected in the cell supernatant (Sup) or within the hydrogel (Gel). HyA-PHT hydrogels (850Pa, 380μM bsp-RGD(15), 0.03wt% heparin, and 40nM TGFβ1) demonstrated significant increases in expression of proteins associated with proliferation and vascular stability relative to identical hydrogels (HyA-P) with an equimolar concentration of soluble TGFβ1 in the media, but without heparin.