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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Biomaterials. 2015 Jan 22;47:1–12. doi: 10.1016/j.biomaterials.2014.12.043

Figure 6. HyA-PHT hydrogels promoted CPC survival and endothelial differentiation in vivo.

Figure 6

a, Bioluminescence post-implantation of GFP-fLuc-mCPCs (~500,000 cells in 100μL gel) in syngeneic mouse hindlimbs. b, Radiance generated by the transplanted CPCs over 32 days (n=5) demonstrated that the hydrogels promoted cell survival and limited cell diffusion away from the implantation site in vivo. c, The transplanted HyA-PHT hydrogels recruited host vessels to the exterior of the implant and a competent vasculature was evident throughout the cross section of the implants. d, Verification of the persistence of donor CPCs with GFP expression, and differentiation of CPCs into endothelial cells was confirmed by expression of CD31. HyA-PHT gels fostered significant and mature blood vessel formation, and infiltration of NG2+ pericytes was observed with the donor GFP+ cells. Scale bars = 100 μm.