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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Biomaterials. 2015 Jan 22;47:1–12. doi: 10.1016/j.biomaterials.2014.12.043

Figure 7. Extracellular matrix production and vascular tone assessed by histology.

Figure 7

a, The HyA-PHT explants exhibited strong collagen staining by Massons’s trichrome, which was rich with Type IV collagen, as visualized by immunohistochemistry. Vascular tone in the explants was evaluated by endomucin staining, and integration of the hydrogels with the host vasculature was assessed by systemic perfusion of the host with Alexa Fluor 568-conjugated GS-IB4 lectin (isolectin). b, Complex vascular structures were observed in the HyA-PHT explants by reconstruction of confocal images of endomucin staining and isolectin perfusion, the latter clearly indicating the vessels within the HyA-PHT hydrogel were anastomosed with the host vessels. Scale bars = 100 μm.