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. Author manuscript; available in PMC: 2016 Jun 21.
Published in final edited form as: Lab Chip. 2015 Jun 21;15(12):2625–2633. doi: 10.1039/c5lc00245a

Figure 4. Delayed retrotaxis during high zymosan-induced burdens.

Figure 4

(A) Quantification of neutrophil trafficking dynamics in the proposed microfluidic assay including the entry and exit rates, and the inflection point at which neutrophils start exiting the CCs and return to blood. (B, C) Neutrophil “Enter” (black), “Exit” (blue) and “Trapped” (red) profiles over time for two different zymosan-induced burdens differentiated based on the number of particles at the CC. At high zymosan particle counts, a high overlap between the “Exit” and “Enter” profiles is observed for at least 6 hours (D) Entry rate is uniform across different zymosan burdens, but significantly higher than the control condition. (E) Inflection time point increases with the increase of the number of zymosan particles at the CC. (F) Exit rate for zymosan experiments decreases with the increase in zymosan count. Difference in exit rate between the three zymosan burden conditions was not significant. Difference between all zymosan burden and control (no zymosan) was significant. *p<0.05, **p<0.01. One-way Analysis of Variance test followed by two-tailed t-test. N = 3 experiments, N > 30 cells/experiment.