Table 1.

TRANSLOCATION MOVEMENTS AND BRANCH FORMATION

“Branches” are the number of branches observed extending from all axons in the imaging field at the beginning of imaging or formed during the imaging period and total of both of these. “Epochs” compare each observed fiber between 2 sequential images, that is, images taken 1 hour apart. (E.g., a fiber that always grew would have 100% growth epochs. One that grew half of the time would have 50% etc. A stable epoch is no observed change of position.) “Net Translocation” is the change in the position of the ending of an axon observed between the first image and the last image of the entire imaging session (5–7 hours). “Gross Movement (Motility)” measures the change in the position of the ending of each axon that occurred between each image (1h intervals) given as an absolute distance (same number regardless of growth or retraction, e.g., 10 μm growth = 10 μm retraction). These distance changes are then summed for each observed axon and averaged for all axons to give “Gross Movement”.

	Vehicle-Treated Fibers	BDNF-Treated Fibers
Branches At Beginning Imaging	0	32*
Branches Formed During Imaging	0	15*
Total Number of Branches	0	47*
Growth Epochs	44%	24%+
Retraction Epochs	33%	24%
Stable Epochs	23%	52%*
Positive Net Translocation	+43 μm (n = 7) (+56 μm)1	+25 μm (n = 24)∘
Negative Net Translocation	−22 μm (n = 4)	−14 μm (n = 18)
Zero Net Translocation	N = 0	N = 16*
Overall Net Translocation	+19 μm	+6 μm
Gross Movement (Motility)	101 μm	26 μm*

^*p = <0.001, two proportion z-test;

⁺p = <0.01, two proportion z-test;

^∘p = <0.05 Mann Whitney rank sum test.

¹Data pooled as per text.