Figure 1. Viral vector plasmid maps and immunohistochemistry.

Plasmid maps for both experimental vectors expressing hemagluttinin (HA)-tagged 5-HT₆ receptors via either the pENK promoter (A) or the pDYN promoter (B). The top six photomicrographs (panels C–H) are of sections from rats infused in dorsal striatum with the pENK-5-HT₆ vector, the bottom six photomicrographs (panels I–N) with pDYN-5-HT₆. Immunohistochemical staining was as follows: leftmost column (C, F, I, L) with primary anti-HA and secondary conjugated Alexa Fluor 488; (D, M) with primary anti-enkephalin and secondary conjugated Alexa Fluor 568; (G, J) with primary anti-substance P and secondary conjugated Alexa Fluor 568. The rightmost column (E, H, K, N) are merges of the two preceding panels. Magnification of figures C–E/I–K is 60x and F–H/L–N is 40x.