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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Autoimmun. 2015 Apr 22;60:40–50. doi: 10.1016/j.jaut.2015.03.005

Figure 6.

Figure 6

Apoptosis in the brain of LPR and LPR KO mice. TUNEL staining (A) was used to evaluate apoptosis in the brains of LPR (n=10), LPR KO (n=8), and MPJ (n=5) mice at 20 weeks of age. Quantitation of the TUNEL staining is shown in (B). Caspase-3 activity assay is shown in (C). The number of mice in the assay for caspase-3 activity in the LPR, LPR KO, and LPR with inhibitor groups was 10, 9, and 10, respectively. Flouro Jade C (FJC) staining was employed to evaluate neuron degeneration in the brain of LPR (n=8), LPR KO (n=6), and MPJ (n=5) mice at 15 weeks of age. Most FJC+ positive cells are located in the cortex (D). The quantitation of cortical FJC staining is shown in (E). TUNEL staining, caspase-3 activity, and FJC staining were repeated twice, with similar results.