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. Author manuscript; available in PMC: 2016 Jul 3.
Published in final edited form as: J Mol Biol. 2015 Feb 27;427(13):2262–2270. doi: 10.1016/j.jmb.2015.02.019

Figure 2. Mapping the transition from water-soluble to membrane-integrated BCL-XL.

Figure 2

(a) Size exclusion chromatography analysis of BCL-XL reconstituted in nanodiscs (in ND), BCL-XL added to preformed nanodiscs (+ND), BCL-XL added to nanodiscs with BIDBH3 (+ND + BIDBH3), or empty nanodiscs (ND). Molecular weights (kDa) of the markers used for calibration are shown at top. (b) Western dot blots of size exclusion chromatography fractions F1 and F2 probed with anti-His antibody to detect BCL-XL, or anti-ApoA1 antibody to detect nanodisc MSP. (c) Selected regions of the solution NMR 1H/15N correlation spectra of water-soluble BCL-XL (blue) and nanodisc-integrated BCL-XL (red). (d) Total difference (ΔHN) in amide 1H and 15N chemical shifts induced by integration in nanodiscs relative to the spectrum of water-soluble BCL-XL.