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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Pathol. 2015 Apr 27;236(3):265–271. doi: 10.1002/path.4533

Figure 2.

Figure 2

(A) p16ink4/HPV16 E2 immunoreactivity in normal-appearing SCJ cells from high-risk women. Some rare Ki67-positive cells were also observed. HPV DNA was not detected by in situ hybridization (ISH). (B) Target cell populations (squamous, columnar and junctional) were detected and microdissected. (C) Total RNA from each cell population was extracted, amplified and HPV16 E6*I, E6*II, E7 and L1 expression was analyzed. HPV E6 and E7 mRNAs were detected in SCJ cells suggesting a transcriptionally active infection in these cells. Internal controls included adjacent squamous and columnar epithelia. Original magnifications: X40 (immunostaining and microdissection) or X200 (bracketed, H&E, ISH).