Figure 2. Curcumin treatment abrogated high glucose-induced ER stress.

Protein levels of p-PERK (A), PERK (A), p-eIF2α (B), eIF2α (B), p-IRE1α (C), IRE1α (C), CHOP (D) and BiP (E) were determined in embryos cultured for 24 h under normal glucose and high glucose conditions, with or without 20 μM curcumin (cur) treatment. Experiments were repeated three times using three embryos from three different dams. NG: normal glucose; NG + Cur: normal glucose plus 20 μM curcumin; HG: high glucose; HG + Cur: high glucose plus 20 mM curcumin. * Indicates significant difference (p < 0.05) when compared to other groups.