Table 1. Curcumin treatment ameliorates high glucose-induced NTD formation.

Group	Total number of embryos	Number of NTD embryos	NTD rate(%)
NG	24	2	8.3
NG + 20μM cur	24	3	12.5
HG	24	13*	54.2
HG + 20μM cur	23	3	13.0
HG + 10μM cur	25	10**	40

NG: normal glucose (100 mg/dl glucose); NG + 20 μM cur: normal glucose plus 20 μM curcumin (cur); HG: high glucose (300 mg/dl glucose); HG + 20 μM cur: high glucose plus 20 μM cur; HG + 10 μM cur: high glucose plus 10 μM cur.

^*Indicates the HG group are significant different when compared with the NG, the NG + 20 μM and the HG + 20 μM groups.

^**Indicates that the HG + 10 μM group and the HG group are not significantly different, and the HG +10 μM group are significantly different when compared with the NG group, the NG + 20 μM group and the HG + 10 μM group. Statistical difference was analyzed by the Chi square test.