Skip to main content
. Author manuscript; available in PMC: 2016 Jul 3.
Published in final edited form as: J Mol Biol. 2015 Apr 16;427(13):2319–2328. doi: 10.1016/j.jmb.2015.04.004

Fig. 4.

Fig. 4

(A) Overlaid aliphatic regions of 2D DARR NMR spectra (500 ms mixing time) showing the effect of isotopic dilution. Gray and red contours correspond to Samples A and B, respectively, where Sample B was isotopically diluted (Table 1). Inter-residue I32/V40 and M35/G37 cross-peaks that were clearly attenuated upon isotopic dilution are marked with blue dashed boxes and green dashed circles, respectively. (B) Horizontal slices taken at the frequencies marked with dashed lines (V40 Cγ slice: 19.4 ppm; M35 Cβ slice: 52.7 ppm) in Panel A. Loss of inter-residue cross-peak intensities with isotopic dilution (between I32 and V40 and between M35 and G37) is visible for the selected signals marked with vertical dotted lines. Slices in Panel B are scaled to the single bond (intra-residue) cross-peaks present in those slices.