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. Author manuscript; available in PMC: 2015 Dec 4.
Published in final edited form as: Nature. 2015 Apr 6;522(7554):94–97. doi: 10.1038/nature14306

Extended Data Fig. 7. Characterization of TGFβ pathway in mesenchymal DP and basal epithelial cell populations during regression.

Extended Data Fig. 7

a) Schematic of skin digestion and cell isolation with representative images before and after tissue digestion, in K14-H2BGFP;Lef1-RFP mice. b) Representative FACS scheme for isolating mesenchymal DP (RFP+, CD34−, CD45−, CD117−, Integrin-alpha 9+) and enriched hair follicle basal epithelium (RFP−, GFPHigh) cells. c) Validation of mesenchymal DP sorted population enrichment by Sox2 expression. d) Validation of basal epithelial sorted population enrichment by keratin-14 (K14) expression. e) TGFβ ligand 2 and 3 expression in the mesenchymal DP throughout the hair cycle. TGFβ-1 expression in basal epithelium, mesenchymal DP, and all sorted cells during regression. f) Differential expression of TGFβ target genes: Smad7, Tmeff1, p15INK4B and in the hair follicle basal epithelium throughout the hair cycle (p<0.005 mean ± SD; n = 3 technical replicates). Scale bars, 100μm.