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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Pathol. 2015 Apr 8;236(3):384–394. doi: 10.1002/path.4530

Fig.1. Reduced TGF-β signaling activity in lung mesenchyme resulted in attenuation of pulmonary inflammation and fibrosis upon bleomycin injury.

Fig.1

(A) Dynamic changes of TβRII, Smad3 protein and activation (phosphorylation) in mouse lung tissues with indicated genotypes after BLM-mediated lung injury. C-Myc is the epitope tag for dnTβRII. GAPDH is a loading control. (B) Sirius Red collagen staining of the mouse lungs with indicated genotypes, which were harvested 28 days after BLM treatment. (C) Histopathology, shown by H&E staining, of the mouse lungs with indicated genotypes at early inflammation stage versus late fibrotic stage. (D) Inflammation was evaluated based on histopathology with scores from 0 (no inflammation) to 15 (worst inflammation). (E) Lung fibrosis was quantified using Ashcroft method. (F) The overall level of lung tissue collagen was measured based on the content of hydroxyproline, presented as hydroxyproline (µg) per mg lung tissue. *P<0.05.