Table 2.
Peak positions and extinction coefficients for CcP, CcP(H52L), metMb, and their imidazole complexes at pH 7.0.
| Proteina | protein band λ (ε) b |
δ band λ (ε) b |
Soret band λ (ε) b |
visible bands λ (ε) b |
|
|---|---|---|---|---|---|
| yCcP | 282 (77) | 374sh (57) | 408 (98) | 508 (11.3) | 646 (3.3) |
| rCcP | 281 (76) | 374sh (60) | 408 (101) | 504 (11.2) | 641 (3.5) |
| H52L | 282 (86) | 379sh (81) | 404 (97) | 511 (12.7) | 651 (4.0) |
| metMb | 280 (39) | 354sh (37) | 409 (188) | 503 (11.3) | 581 (4.6) |
| yCcP·imidazole | - | - | 412 (85) | 542 (14.7) | 570 (12.9) |
| rCcP·imidazole | - | 360sh (31) | 412 (109) | 540 (10.7) | 574sh (7.9) |
| metMb-imidazole | 280sh (36) | 361 (32) | 415 (146) | 535 (12.9) | 365sh (9.1) |
| yCcP·MIM | - | 350 (28) | 416 (131) | 542 (13.3) | 572 (11.9) |
| rCcP·MIM | - | 346 (20) | 420 (121) | 544 (12.4) | 574sh (10.1) |
| H52L-MIM | - | 420 (133) | 544 (13.7) | 572sh (12.8) | |
| metMb·MIM | 280sh (35) | 357 (28) | 417 (141) | 534 (13.2) | 562sh (9.8) |
a
Abbreviations: MIM, 1-methylimidazole.
b
λ, wavelength in nm. ε, extinction coefficient in mM−1 cm−1.