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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Surg Res. 2015 Mar 18;197(1):126–138. doi: 10.1016/j.jss.2015.03.023

Figure 6. Effect of HB-EGF on STAT3 activation during HB-EGF macrophage polarization.

Figure 6

(A) Silencing efficiency of STAT3 siRNA in macrophages as determined by real-time PCR. *p<0.05 vs. scrambled siRNA. (B) Macrophages derived from THP-1 cells were transfected with either scrambled control siRNA or STAT3 siRNA and were then exposed to the indicated treatments for 24h. M2 macrophages were labeled with PE-Cy5-conjugated anti-human CD206 and APC-conjugated anti-human F4/80 antibodies and then analyzed the percentage of M2 macrophages/total macrophages by flow cytometry. Representative FACS plots are shown. Gating was performed on CD206+/F4/80+ cells. (C) Quantification of data from B. * p<0.05 vs. scramble siRNA transfected MØ+HB-EGF; † p<0.05 vs. scramble siRNA transfected MØ+HB-EGF+LPS. (D) mRNA expression of M2 associated markers was examined using real time PCR. The transcript level of each gene was normalized against the level of unstimulated macrophages, which was arbitrarily set as 1. Data represent the mean ± SD of four independent experiments.

* p<0.05 vs. scramble siRNA transfected MØ+HB-EGF; † p<0.05 vs. scramble siRNA transfected MØ+HB-EGF+LPS.

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