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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Surg Res. 2015 Mar 18;197(1):126–138. doi: 10.1016/j.jss.2015.03.023

Figure 7. Effect of HB-EGF on macrophage infiltration and polarization during NEC.

Figure 7

(A) Representative immunofluorescent staining for the total macrophage marker CD68 (red) and the M1 macrophage marker CD86 (green) of intestinal sections obtained from mouse pups exposed to breast feeding (n=12), NEC (n=12), or NEC + HB-EGF (n=12). Nuclei are indicated by DAPI staining. M1 macrophages are indicated by co-localization of CD68 and CD86 in the merged images. (B) Representative immunofluorescent staining for the total macrophage marker CD68 (red) and the M2 macrophage marker CD206 (green). Nuclei are indicated by DAPI staining. M2 macrophages are indicated by co-localization of CD68 and CD206 in the merged images. In A and B, eight segments of intestine were analyzed for each mouse pup, and four fields in each section were viewed and counted blindly by two independent investigators. (C) Quantification of the results from A and B. Data represent mean ± SD. * p<0.01 vs. BF; **<0.05 vs. NEC; †p<0.05 vs. BF; ††p<0.05 vs. NEC; ‡ p<0.05 vs. NEC. (D) Quantification of the percent of macrophage subtypes in intestinal sections from mouse pups exposed to the indicated treatments. Data represent mean ± SD. Statistical analysis was performed with ANOVA followed by Bonferroni’s multiple comparison test.

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