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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Struct Biol. 2015 May 5;190(3):279–290. doi: 10.1016/j.jsb.2015.04.016

Figure 4. Reference-free iterative refinement of cryoET-ZPC ε15 subtomograms with and without symmetry.

Figure 4

A) Symmetry-free average of 86 ε15 subtomograms. B) Average of 66 ε15 subtomograms refined with icosahedral symmetry enforced. C) Orthogonal projections of the structure in A). D) Orthogonal projections of the structure in B). E) Gold-standard FSC curves for the maps in A) (teal) and B) (brown), indicating ~37 Å and ~24 Å resolution at the 0.143 cutoff.