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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Struct Biol. 2015 May 5;190(3):279–290. doi: 10.1016/j.jsb.2015.04.016

Figure 6. Reference-free D7 GroEL subtomogram average.

Figure 6

A) Average of 226 GroEL subtomograms iteratively refined imposing D7 symmetry, shown as an isosurface (left, blue) and super imposed with the known crystal structure (right, pink; PDB ID 3CAU) converted to EM density and filtered to the same resolution. B) Corresponding orthogonal projections of the map in A). C) Gold standard FSC curve for the map in A) indicating ~24 Å resolution.