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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2015 Apr 4;84:196–205. doi: 10.1016/j.freeradbiomed.2015.03.036

Figure 5. Hyperoxia-mediated lung edema and alveolar leak were suppressed in Ad-SOCS-1-treated mice and Ad-SOCS-1 induced alveolar fluid clearance in mice exposed to hyperoxia.

Figure 5

Male and female C57Bl/6 mice transfected with recombinant Ad-SOCS-1 and Ad-GFP were exposed to room air (Normoxia) or 100% O2 (Hyperoxia) at 72 h as described in Material and Methods. (A) Total protein content and (B) lung edema were determined by BCA assay, wet/dry ratio, and spectrophotometric assay, respectively, in Ad-SOCS-1-treated mice and Ad-GFP-treated mice exposed to room air or hyperoxic conditions. (C) Ad-SOCS-1- or Ad-GFP-transfected mice were exsanguinated and alveolar fluid clearance was measured by tracheal instillation of I131 albumin. *P < 0.05 when compared between Ad-GFP- and Ad-SOCS-1-transfected mice under hyperoxia.