Table 1.

Representative list of differentially expressed genes by melatonin in MCF-7 cells

GENE SYMBOL	GENE NAME	FOLD CHANGE	FUNCTION	ACCESSION NUMBER
Cell-to-cell adhesion and communication
FN1	Fibronectin 1	−2.6	Involves cell adhesion, cell motility, wound healing. Possible role in metastasis	AW385690
DPI, DPII	Desmoplakin (DPI, DPII)	−2.3	Intercellular junction, adhesion protein	M77830
Cytokine and cytokine receptors
BMP7	Bone morphogenetic protein 7 (osteogenic protein 1)	−2.5	Member of the transforming growth factor-beta superfamily, induces epithelial cell growth	BE395650
Growth factor and receptors
AREG	Amphiregulin (schwannoma-derived growth factor)	−2.0	A member of the epidermal growth factor family, growth inhibition and stimulations	NM_001657
IGF1R	Insulin-like growth factor 1 receptor	−1.4	Binds insulin-like growth factor, plays a critical role in transformation events.	X04434
Signal transduction-related
S100P	S100 calcium-binding protein P	−2.6	Calcium-binding protein, mediates Ca2+ signals, overexpressed in breast cancer	BE536069
CALR	Calreticulin	1.6	Ca2+ binding protein, modulator of nuclear receptor function, possible role in angiogenesis	M84739
NUCB2	Nucleobindin 2	2.8	Ca2+ binding protein, possible role in calcium homeostasis	AF052644
Chromatin remodeler and coactivator
CITED2	Cbp/p300-interacting transactivator	minus;1.7	Coactivator of transcription factors	BF110899
SRC1	Nuclear receptor coactivator 1	−1.4	Transcriptional coactivator for steroid and nuclear hormone receptors.	U59302
Transcription factor
EPAS1	Endothelial PAS domain protein 1	−2.3	Transcriptional factor, induces VEGF expression, possible role in angiogenesis	AW377189
Cell proliferation-related
Ki-67	Antigen identified by monoclonal antibody Ki-67	−1.8	Possible role in cell proliferation	X65550
p21	Cyclin-dependent kinase inhibitor 1A (p21, Cip1)	1.7	Cyclin-dependent kinase inhibitor, blocking cell cycle progression	U09579
Development and differentiation-related
NDRG1	N-myc downstream regulated	−2.1	A member of the N-myc downregulated gene family, possible role in growth arrest and cell differentiation, induced by hypoxia	X92845
Metastasis-related
IGFBP5	Insulin-like growth factor binding protein 5	−3.3	Growth inhibitor and proapoptotic agent in breast cancer, possible role in metastasis	AA374325
FN1	Fibronectin 1	−2.6	Cell adhesion, cell motility, wound healing. Possible role in metastasis	AW385690
EphA1	EphA1	−1.6	Ephrin receptor subfamily of the protein-tyrosine kinase family, possible role in metastasis	Z27409
TIMP3	Tissue inhibitor of metalloproteinase 3	1.9	Natural inhibitors of the matrix metalloproteinases, inhibits VEGF binding to VEGFR2 and angiogenesis	AI095372
Angiogenesis
EPAS1	Endothelial PAS domain protein 1	−2.3	Transcriptional factor, induces VEGF expression, possible role in angiogenesis	AW377189
NDRG1	N-myc downstream regulated	−2.1	A member of the N-myc downregulated gene family, induced by hypoxia	X92845
FN1	Fibronectin 1	−2.6	Involves cell adhesion, cell motility, wound healing. Possible role in metastasis	AW385690
EphA1	EphA1	−1.6	Ephrin receptor subfamily of the protein-tyrosine kinase family, possible role in metastasis	Z27409
CALR	Calreticulin	1.6	Ca2+ binding protein, modulator of nuclear receptor function, possible role in angiogenesis	M84739
TIMP3	Tissue inhibitor of metalloproteinase 3	1.9	Natural inhibitors of the matrix metalloproteinases, inhibits VEGF binding to VEGFR2 and angiogenesis	AI095372

^*Positive number of fold change represents the up-regulation of gene expression by melatonin.

^*Negative number of fold change represents the down-regulation of gene expression by melatonin.