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. 2015 Jun 5;10(6):e0129314. doi: 10.1371/journal.pone.0129314

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© 2015 Ghaleb et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — A. Quantification of positive cells as a result of IF shows significantly decreased numbers of infiltrating macrophages (F4/80), neutrophils (Ly-6G) and dendritic cells (CD11c) in Iqgap2 ^-/- colons compared to WT following DSS treatment. The number of positive fluorescent cells was obtained by counting cells in six randomly selected fields per colon sample from WT and Iqgap2 ^-/- mice from the three groups: untreated, treated with DSS, and treated with DSS and allowed to recover for 7 days. B. Complete blood count (CBC) confirms reduced numbers of neutrophils, lymphocytes and monocytes in circulation in Iqgap2 ^-/- mice treated with DSS. Data are presented as the mean ± SEM. N = 3 per genotype/treatment (A), N = 5 per genotype/treatment (B), p-values indicating statistically significant differences are shown.