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. Author manuscript; available in PMC: 2015 Jun 6.
Published in final edited form as: J Bone Miner Res. 2014 Aug;29(8):1872–1885. doi: 10.1002/jbmr.2213

Figure 2. Dose-dependent activation of alkaline phosphatase activity by oxysterols.

Figure 2

Figure 2

(A) C3HT101/2 cells or (B) M2-10B4 cells at confluence were treated with control vehicle or 0.125-10 μM of Oxy133. For direct comparison to Oxy133, C3H cells were also treated with Oxy34 and Oxy49 (A). After 4 days, alkaline phosphatase (ALP) activity was measured in whole cell extracts. Data from a representative of three separate experiments are reported as the mean of triplicate determinations ± SD and normalized to protein concentration. (p <0.0001 for cells treated with 0.25 μM or higher dose of all oxysterols vs. control vehicle treated cells).