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. 2015 Jun 5;10(6):e0128693. doi: 10.1371/journal.pone.0128693

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© 2015 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) The alterations of cell morphology were analyzed by flow cytometry. FSC (y-axis) is an indicator of size, SSC (x-axis) is an indicator of granularity and the z-axis represents the cellular population intensity. The values of x-axis and y-axis are linear and provide relative values for comparison among different treatments. (B) The contents of intracellular glycerol were measured using Glycerol Assay Kits after treated with various doses of DD for 3 h. (C) The total RNAs were extracted by the hot phenol method and the expressions of HOG1 and RHR2 were measured using qPCR. Bars indicate standard deviations.