Table 1. Molecular weight of FLAG-BRI2 peptide detected in FBA/SPP co-transfected cell lysates.
| Sequence | MW Calculated (Da) | MW Observed (Da) | ||
|---|---|---|---|---|
| SPPL2b | hSPP | pSPP | ||
| FLAG-MVKV…GQRRAWCWCMCFGLAFMLAGV | 8524 | 8523 (-1) | ||
| FLAG-MVKV…GQRRAWCWCMCFG | 7720 | 7762(+42)*7803(+83) | 7761 (+41)7804 (+84) | |
| FLAG-MVKV…GQRRAWCWCMCF | 7664 | 7663 (-1)7706(+42) | 7663 (-1)7706 (+42) | |
| FLAG-MVKV…GQRRAWCWCMC | 7517 | 7558(+41)7601(+84) | 7558 (+41)7602 (+85) | |
| FLAG-MVKV…GQRRAWCWCM | 7413 | 7455(+42)7497(+84) | 7456 (+43)7497 (+84) | |
| FLAG-MVKV…GQRRAWCWC | 7282 | 7325 (+43)7366 (+84) | ||
*The number in the brackets is the difference between observed and calculated MW. Error between observed and calculated mass is less than ±0.1%.