Fig 4. HIF-1α expression in CD11c⁺ cells limits expansion of CD8⁺ T cells.

(A-B) Percentage of in vitro infected Hif-1α ^flox/flox Cd11c-Cre ^- and Hif-1α ^flox/flox Cd11c-Cre ⁺ BMDC (A) and CD11c⁺ cells (B). (C) 2x10⁴ OT-I CD8⁺ T cells were adoptively transferred into recipient mice a day prior to infection with 2x10⁷ovalbumin-transgenic (PINK) L. donovani amastigotes. Graph represents the average number of OT-I CD8⁺ T cells found in the spleen from Hif-1α ^flox/flox Cd11c-Cre ⁺ and Cre ^- at d7 p.i. (left panel) and d14 p.i. (right panel). (D) Percentage of OT-I CD8⁺ T cells at d7 p.i. (left panel) and d14 p.i. (right panel). All data is presented as the mean ± SEM of one of three independent experiments, n = 5. * denotes p<0.05, ** denotes p<0.01 and *** denotes p<0.001.