Table 1. Synthetic peptides of the IBDV VP4 protein in this study.
| Peptide name | Amino acid sequence |
|---|---|
| Pep533-549pSer538 | 533cDGILAS*PGVLRGAHNLD549 |
| Pep533-549 | 533cDGILASPGVLRGAHNLD549 |
| Pep602-619pTyr611 | 602cTLSGHRVYGY*APGGVLP619 |
| Pep602-619 | 602cTLSGHRVYGYAPGGVLP619 |
| Pep667-683Thr674 | 667cVPIHVAMT*GALNA683 |
| Pep667-683 | 667cVPIHVAMTGALNA683 |
| Pep515-558 | 515cKGYEVVANLFQVPQNPVVDGILASPGVLRGAHNLDCVLREGATL558 |
| Pep550-565 | 550CVLREGATLFPVVITT565 |
| Pep563-600 | 563cITTVEDAMTPKALNSKMFAVIEGVREDLQPPSQRGSF600 |
| Pep598-631 | 598cSFIRTLSGHRVYGYAPGGVLPLETGRDYTVVPID631 |
| Pep618-641 | 618cPLETGRDYTVVPIDDVWDDSIMLS641 |
| Pep638-662 | 638cIMLSKDPIPPIVGNSGNLAIAYMDV662 |
| Pep653-674 | 653cGNLAIAYMDVFRPKVPIHVAMT674 |
| Pep680-706 | 680CGEIEKVSFRSTKLATAHRLGLKLAGP706 |
| Pep700-717 | 700cGLKLAGPGAFDVNTGPNW717 |
| Pep713-741 | 713cTGPNWATFIKRFPHNPRDWDRLPYLNLPY741 |
| Pep720-750 | 720cFIKRFPHNPRDWDRLPYLNLPYLPPNAGRQY750 |
| Pep515-532 | 515cKGYEVVANLFQVPQNPVV532 |
| Pep524-558 | 524cFQVPQNPVVDGILASPGVLRGAHNLDCVLREGATL558 |
| Pep533-558 | 533cDGILASPGVLRGAHNLDCVLREGATL558 |
| Pep524-539 | 524cFQVPQNPVVDGILASP539 |
| Pep534-549 | 534cGILASPGVLRGAHNLD549 |
| Pep543-558 | 543cRGAHNLDCVLREGATL558 |
| Pep534-542 | 534cGILASPGVL542 |
| Pep524-534 | 524cFQVPQNPVVDG534 |
| Pep524-528 | 524cFQVPQ528 |
| Pep529-534 | 529cNPVVDG534 |
| Pep526-532 | 526cVPQNPVV532 |
| Pep530-536 | 530cPVVDGIL536 |
| Pep531-535 | 531cVVDGI535 |
| Pep531-536 | 531cVVDGIL536 |
| Pep532-536 | 532cVDGIL536 |
| Pep533-536 | 533cDGIL536 |
| Pep530-535 | 530cPVVDGI535 |
| Pep530-534 | 530cPVVDG534 |
| Pep530-536P530A | 530cAVVDGIL536 |
| Pep530-536V531A | 530cPAVDGIL536 |
| Pep530-536V532A | 530cPVADGIL536 |
| Pep530-536D533A | 530cPVVAGIL536 |
| Pep530-536G534A | 530cPVVDAIL536 |
| Pep530-536I535A | 530cPVVDGAL536 |
| Pep530-536L536A | 530cPVVDGIA536 |
Note:
“*” indicates the amino acids with phosphorylation.
The letter “c” means cysteine appended to the Sulfo-SMCC cross-linker. Mutated residues are underlined.