Table 4. The summary of proteolytic cleavage result affected by a series of substitution in this study.
mutation type | Ala-substitution | Asp- substitution | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
position | S538 | Y611 | T674 | S538Y611 | S538T674 | Y611T674 | S538Y611T674 | S538 | Y611 | T674 | S538Y611 | S538T674 | Y611T674 | S538Y611T674 |
result | + | + | - | + | - | - | - | + | - | - | - | - | - | - |
Note:
“+” means such mutation does not affect intermediate precursor VP4-VP3 cleavage;
“-” means such mutation affects intermediate precursor VP4-VP3 cleavage.