Table 1. List of genes with their respective primers for GeXP multiplex analysis.
Gene | Forward primer sequence | Reverse primer sequence |
---|---|---|
Bcl-2 | 5’-ACCACTAATTGCCAAGCACC-3’ | 3’-ATTTTCCATCCGTCTGCTCTT-5’ |
Bax | 5’-CCCTTTTGCTTCAGGGTTTC-3’ | 3’-ACAAAGTAGAAAAGGGCGACAA-5’ |
p21 | 5’-TTAGCAGCGGAACAAGGAGT-3’ | 3’-AAGCCGAGAGAAAACAGTCCA-5’ |
PARP1 | 5’-TATCGAGTCGAGTACGCCAA-3’ | 3’-AAACTACCTTTTCAGGGTGTG-5’ |
Beta actin | 5’-GATCATTGCTCCTCCTGAGC-3’ | 3’-AAAAGCCATGCCAATCTCATC-5’ |
Kan(r)b | 5’-ATCATCAGCATTGCATTCGAT TCCTGTTTG-3’ | 3’-AATTCCGACTCGTCCAACATC-5’ |
Forward universal primer sequence (5’-AGGTGACACTATAGAATA-3’); Reverse universal primer sequence (3’-GTACGACTCACTATAGGG-5’).