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. 2015 Jun 5;10(6):e0128788. doi: 10.1371/journal.pone.0128788

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© 2015 Prabudiansyah et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — A) Overexpression of M. tuberculosis SecA1 (lane 2) and SecA2 (lane 4) in E. coli BL21(λDE3) with a molecular mass of 105 and 85 kDa, respectively. Molecular masses of protein standard are indicated on the left. The empty vector, pACYCDuet-1 and pET15b, are shown as controls (lane 1 and 3). B) Coomassie-stained SDS-PAGE of purified M. tuberculosis SecA1 (lane 1) and SecA2 (lane 2). C) Visualization of fluorescently labeled SecA1 and SecA2 in SDS-PAGE by fluorescence imaging. SecA1 and SecA2 were labeled with the fluorescent probe Cy5 (lane 1 and 2) or AF488 (lane 3 and 4).