Fig 7. Increased de novo lipogenesis in Glmp ^gt/gt hepatocytes.

Overnight cultured primary hepatocytes from WT and Glmp ^gt/gt mice were incubated with [¹⁴C]acetate (0.5 μCi/mL, 100 μM) for 4 h. (A) The amount of synthesized non-esterified fatty acids (NEFA), (B) diacylglycerol (DAG), (C) triacylglycerol (TAG), (D) phospholipids, (E) cholesterol esters (CE) and (F) total lipids were analyzed using thin layer chromatography (n = 8 experiments, representing 4 individual mice/genotype, with 2 replicates each, * p < 0.05 vs. WT). Values are presented as mean ± SEM.