Fig 2. CAI reduced vascular calcification in rats.

Rats were treated with high dose of vitamin D₂ (Control) with or without high cholesterol diet (HCD) in the presence or absence of CAI. The parameters were measured as described in Materials and Methods. A: Calcium deposition in aorta arch in brown yellow color (A1: representative photograph, black arrow indicates the calcium deposition; A2: statistical results); B: Calcium content in aortic tissue; C: DHE in red color indicating superoxide anion (C1: representative photograph; C2: statistical results); D: Serum content of total cholesterol (TC); E: oxLDL content in serum. Results were expressed as mean±S.D. n = 8. *: P<0.05 was considered statistically significant.