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. 2015 Jun 5;10(6):e0129128. doi: 10.1371/journal.pone.0129128

Fig 8. Inhibition of ROS in mitochondria reduced calcification of RVSMCs and regulated the expression of ALP.

Fig 8

In one experiment, RVSMCs were treated with or without oxLDL in the presence or absence of calpain-1 siRNA. In the other experiments, RVSMCs were treated with or without oxLDL in the presence or absence of TEMPO. The parameters were measured as described in Materials and Methods. A: Mito-SOX in red color indicating ROS production in mitochondria of RVSMCs (A1: representative photograph; A2: statistical results); B: Calcium deposition in RVSMCs in red and black color (B1: representative photograph; B2: statistical results); C: PPi content in medium; D: ALP activity in medium; E: ALP activity in RVSMCs; F: ALP mRNA expression in RVSMCs; G: ALP protein expression in RVSMCs (upper: representative photograph; lower: statistical results). Results were expressed as mean±S.D. n = 4. *: P<0.05 was considered statistically significant.