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. 2015 Jun 5;10(6):e0128709. doi: 10.1371/journal.pone.0128709

Fig 2. A: Testicular histopathology from normal (N, nonimmunized) and Experimental (E) rats (immunized with TH and adjuvants), injected with saline solution (E+saline) or L-NAME (8mg/kg) (E+L-NAME) killed 50 days after the first immunization.

Fig 2

A) Seminiferous tubules (ST) with normal spermatogenesis in N rats. Testis section from a rat of E+saline group, shows all ST with different degrees of germ cell sloughing and moderate interstitial lymphomononuclear infiltrates (focal EAO). ST with moderate germ cell sloughing intermingled with ST with normal spermatogenesis were observed in rats from E+L-NAME group. Scale bar indicates 100μm. B) EAO score of testes from rats of N (n = 9), E+saline (n = 11) or E+L-NAME (n = 12), groups. Horizontal lines represent the mean, the dotted line indicates EAO score for values above 2.5. Each symbol represents a single rat. EAO score (mean±SEM): N: 0.111 ± 0.09, EAO+saline: 4.273 ± 0.821, E+L-NAME: 1.667, p<0.01. Student t Test. C) % of ST with apoptotic germ cells (GC, TUNEL technique). Each bar represents mean±SEM. **p<0.01 vs N and Δp<0.05 vs E+saline. Bonferroni Multiple Comparison Test. D) Nitrite content was evaluated in the testis of N, E+saline and E+L-NAME rats. Each bar represents mean±SEM. **p<0.01 vs N and Δp<0.05 vs E+saline. Bonferroni Multiple Comparison Test.